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  • KD/KO Validated

PLEKHO1 Polyclonal antibody

PLEKHO1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24883-1-AP

Synonyms

C Jun binding protein, CK2 interacting protein 1, CKIP 1, CKIP1, CKIP-1, HQ0024c, JBP, OC120, PLEKHO1



Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24883-1-AP targets PLEKHO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHO1 fusion protein Ag19309
Full Name pleckstrin homology domain containing, family O member 1
Calculated Molecular Weight 409 aa, 46 kDa
Observed Molecular Weight 46 kDa, 90 kDa
GenBank Accession NumberBC010149
Gene Symbol PLEKHO1
Gene ID (NCBI) 51177
RRIDAB_2879778
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLEKHO1, also named as CK2 interacting protein 1 or OC120, is a 409 amino acid protein, which contains one PH domain. PLEKHO1 Predominantly localizes to the plasma membrane. PLEKHO1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). Catalog#24883-1-AP recognizes 46 kDa and 90 kDa homodimer band.

Protocols

Product Specific Protocols
WB protocol for PLEKHO1 antibody 24883-1-APDownload protocol
IHC protocol for PLEKHO1 antibody 24883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

J Dent Res

Ckip-1 Mediates P. gingivalis-Suppressed Cementoblast Mineralization.

Authors - X Huang
  • KD Validated
mouseWB

Front Physiol

Myocardial CKIP-1 Overexpression Protects from Simulated Microgravity-Induced Cardiac Remodeling.

Authors - Shukuan Ling
mouseWB

Front Physiol

Casein Kinase-2 Interacting Protein-1 Regulates Physiological Cardiac Hypertrophy via Inhibition of Histone Deacetylase 4 Phosphorylation.

Authors - Yinlong Zhao
ratWB

Front Pharmacol

Exploratory study of sea buckthorn enhancing QiangGuYin efficacy by inhibiting CKIP-1 and Notum activating the Wnt/β-catenin signaling pathway and analysis of active ingredients by molecular docking

Authors - Yi-Feng Yuan
mouseWB

J Oral Microbiol

CKIP-1 mediates P. gingivalis-suppressed osteogenic/cementogenic differentiation of periodontal ligament cells partially via p38 signaling pathway

Authors - Xin Huang