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PLEKHO2 Polyclonal antibody

PLEKHO2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 21356-1-AP

Synonyms

PLEKHO2, PLEKHQ1, PP1628, pp9099

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PLEKHO2 Polyclonal antibody (21356-1-AP)

    WB analysis of HeLa using 21356-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21356-1-AP (PLEKHO2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse thymus tissue using PLEKHO2 Polyclonal antibody (21356-1-AP)

    WB analysis of mouse thymus using 21356-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21356-1-AP (PLEKHO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PLEKHO2 Polyclonal antibody (21356-1-AP)

    IHC staining of mouse spleen using 21356-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21356-1-AP (PLEKHO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PLEKHO2 Polyclonal antibody (21356-1-AP)

    IF Staining of A431 using 21356-1-AP

    Immunofluorescent analysis of A431 cells using 21356-1-AP (PLEKHO2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse thymus tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21356-1-AP is PLEKHO2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHO2 fusion protein Ag15786
Full Name pleckstrin homology domain containing, family O member 2
Calculated Molecular Weight 490 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC008744
Gene Symbol PLEKHO2
Gene ID (NCBI) 80301
RRIDAB_2878846
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pleckstrin homology domain-containing proteins family O member 2 (PLEKHO2)'s function remains largely unknown. Genetic polymorphisms of PLEKHO2 contribute to diabetic retinopathy (DR) development. Functional prediction analysis strengthened the likelihood of participation of PLEKHO2 in the development of DR (Eric C. Han et al., 2012).

Protocols

Product Specific Protocols
WB protocol for PLEKHO2 antibody 21356-1-APDownload protocol
IHC protocol for PLEKHO2 antibody 21356-1-APDownload protocol
IF protocol for PLEKHO2 antibody 21356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols