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  • KD/KO Validated

PLOD2 Monoclonal antibody

PLOD2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H9E1

Cat no : 66342-1-Ig

Synonyms

LH2, Lysyl hydroxylase 2, PLOD2, TLH



Tested Applications

Positive WB detected inDU 145 cells, A431 cells, HEK-293 cells, A549 cells, Hela cells, HEK293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66342-1-Ig targets PLOD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen PLOD2 fusion protein Ag5779
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Calculated Molecular Weight 758 aa, 85 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC037169
Gene Symbol PLOD2
Gene ID (NCBI) 5352
RRIDAB_2881722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy.(PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for PLOD2 antibody 66342-1-IgDownload protocol
IHC protocol for PLOD2 antibody 66342-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Cell Dev Biol

Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.

Authors - Yajuan Zhao
humanWB

J Invest Dermatol

Loss of RhoE function in dermatofibroma promotes disorganized dermal fibroblast extracellular matrix and increased integrin activation

Authors - Sofia Endzhievskaya
  • KD Validated
humanWB,IHC

Cell Death Dis

Hypoxia-induced PLOD2 promotes clear cell renal cell carcinoma progression via modulating EGFR-dependent AKT pathway activation

Authors - Tao Liu
  • KD Validated