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  • Featured Product
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PLRG1 Polyclonal antibody

PLRG1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11914-1-AP

Synonyms

Pleiotropic regulator 1, PLRG1, PRL1

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PLRG1 Polyclonal antibody (11914-1-AP)

    WB analysis of K-562 using 11914-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PLRG1 Polyclonal antibody (11914-1-AP)

    WB analysis of HEK-293 using 11914-1-AP

    WB result of PLRG1 antibody (11914-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLRG1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using PLRG1 Polyclonal antibody (11914-1-AP)

    WB analysis of HEK-293 using 11914-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using PLRG1 Polyclonal antibody (11914-1-AP)

    WB analysis of BxPC-3 using 11914-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PLRG1 Polyclonal antibody (11914-1-AP)

    IP experiment of HEK-293 using 11914-1-AP

    IP result of anti-PLRG1 (IP:11914-1-AP, 4ug; Detection:11914-1-AP 1:1000) with HEK-293 cells lysate 1400ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using PLRG1 Polyclonal antibody (11914-1-AP)

    IHC staining of human brain using 11914-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using PLRG1 Polyclonal antibody (11914-1-AP)

    IHC staining of human brain using 11914-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, BxPC-3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11914-1-AP targets PLRG1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLRG1 fusion protein Ag2527
Full Name pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
Calculated Molecular Weight 514 aa, 57 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC020786
Gene Symbol PLRG1
Gene ID (NCBI) 5356
RRIDAB_2165608
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLRG1, identifed as a component of the CDC5L complex, is one spliceosomal protein highly conserved across species. It has been shown to be essential for pre-mRNA splicing in yeast and humans.

Protocols

Product Specific Protocols
WB protocol for PLRG1 antibody 11914-1-APDownload protocol
IHC protocol for PLRG1 antibody 11914-1-APDownload protocol
IP protocol for PLRG1 antibody 11914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.

Authors - Shuai Hou
View Article
humanWB

Cell Death Differ

USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis.

Authors - Shuyan Liu
View Article