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PMF1 Polyclonal antibody

PMF1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11190-2-AP

Synonyms

PMF 1, PMF1, polyamine modulated factor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PMF1 Polyclonal antibody (11190-2-AP)

    WB analysis of Jurkat using 11190-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11190-2-AP (PMF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PMF1 Polyclonal antibody (11190-2-AP)

    WB analysis of HepG2 using 11190-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11190-2-AP (PMF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PMF1 Polyclonal antibody (11190-2-AP)

    IHC staining of human gliomas using 11190-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11190-2-AP (PMF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • PMF1 Antibody IHC human gliomas tissue 11190-2-AP

    IHC staining of human gliomas tissue using 11190-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11190-2-AP( PMF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PMF1 Polyclonal antibody (11190-2-AP)

    IF Staining of HepG2 using 11190-2-AP

    Immunofluorescent analysis of HepG2 cells using 11190-2-AP (PMF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11190-2-AP targets PMF1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMF1 fusion protein Ag1672
Full Name polyamine-modulated factor 1
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC033656
Gene Symbol PMF1
Gene ID (NCBI) 11243
RRIDAB_2064906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PMF1 antibody 11190-2-APDownload protocol
IHC protocol for PMF1 antibody 11190-2-APDownload protocol
IF protocol for PMF1 antibody 11190-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols