Tested Applications
| Positive WB detected in | Jurkat cells, HepG2 cells, HeLa cells, K-562 cells, MOLT-4 cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28186-1-AP targets PMS1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28132 Product name: Recombinant human PMS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 340-616 aa of BC096331 Sequence: LPSTNSYENNKTDVSAADIVLSKTAETDVLFNKVESSGKNYSNVDTSVIPFQNDMHNDESGKNTDDCLNHQISIGDFGYGHCSSEISNIDKNTKNAFQDISMSNVSWENSQTEYSKTCFISSVKHTQSENGNKDHIDESGENEEEAGLENSSEISADEWSRGNILKNSVGENIEPVKILVPEKSLPCKVSNNNYPIPEQMNLNEDSCNKKSNVIDNKSGKVTAYDLLSNRVIKKPMSASALFVQDHRPQFLIENPKTSLEDATLQIEELWKTLSEEE Predict reactive species |
| Full Name | PMS1 postmeiotic segregation increased 1 (S. cerevisiae) |
| Observed Molecular Weight | 106 kDa |
| GenBank Accession Number | BC096331 |
| Gene Symbol | PMS1 |
| Gene ID (NCBI) | 5378 |
| RRID | AB_2881083 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54277 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PMS1 antibody 28186-1-AP | Download protocol |
| IP protocol for PMS1 antibody 28186-1-AP | Download protocol |
| WB protocol for PMS1 antibody 28186-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
valentina (Verified Customer) (12-11-2025) | used for the detection of recombinant PMS1. When the human MLH1-PMS1 complex was used as pray in pull down experiments (1 ug of recombinant MutLalpha complex was incubated with 50 ul of amylose resin coated with MBP-tagged protein used as a bait) western blot analysis of the result was obtained within a few seconds of exposure.
|
Daniela (Verified Customer) (03-17-2022) | The staining was aspecific and very weak.
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