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PNCK Polyclonal antibody

PNCK Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 13712-1-AP

Synonyms

BSTK3, CaM KI beta, CaM kinase I beta, CaM kinase IB, CaMK1b, CaMKI beta, PNCK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PNCK Polyclonal antibody (13712-1-AP)

    WB analysis using 13712-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13712-1-AP (PNCK antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PNCK Polyclonal antibody (13712-1-AP)

    WB analysis of human brain using 13712-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13712-1-AP (PNCK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using PNCK Polyclonal antibody (13712-1-AP)

    IHC staining of human ovary using 13712-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PNCK Polyclonal antibody (13712-1-AP)

    IHC staining of human ovary using 13712-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13712-1-AP targets PNCK in WB, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNCK fusion protein Ag4650
Full Name pregnancy up-regulated non-ubiquitously expressed CaM kinase
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 38 kDa, 45 kDa
GenBank Accession NumberBC033746
Gene Symbol PNCK
Gene ID (NCBI) 139728
RRIDAB_10642952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PNCK antibody 13712-1-APDownload protocol
IHC protocol for PNCK antibody 13712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols