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PNKP Polyclonal antibody

PNKP Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13558-1-AP

Synonyms

DNA 5 kinase/3 phosphatase, PNK, PNKP

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PNKP Polyclonal antibody (13558-1-AP)

    WB analysis of Jurkat using 13558-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13558-1-AP (PNKP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PNKP Polyclonal antibody (13558-1-AP)

    WB analysis of HeLa using 13558-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13558-1-AP (PNKP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PNKP Polyclonal antibody (13558-1-AP)

    IHC staining of human breast cancer using 13558-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PNKP Polyclonal antibody (13558-1-AP)

    IHC staining of human breast cancer using 13558-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13558-1-AP targets PNKP in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNKP fusion protein Ag4421
Full Name polynucleotide kinase 3'-phosphatase
Calculated Molecular Weight 521 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC033822
Gene Symbol PNKP
Gene ID (NCBI) 11284
RRIDAB_2877958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PNKP antibody 13558-1-APDownload protocol
IHC protocol for PNKP antibody 13558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Isabelle Cristine (Verified Customer) (01-14-2022)

This antibody works extremely well for detecting PNKP in HEK293 cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293
PNKP Antibody Western Blot validation (1:500 dilution) in HEK293 (Cat no:13558-1-AP)