POFUT2 Polyclonal antibody

POFUT2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17764-1-AP

Synonyms

C21orf80, FUT13, KIAA0958, O FucT 2, Peptide O fucosyltransferase 2, POFUT2, protein O fucosyltransferase 2



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells
Positive IHC detected inmouse liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17764-1-AP targets POFUT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POFUT2 fusion protein Ag12148
Full Name protein O-fucosyltransferase 2
Calculated Molecular Weight 429 aa, 50 kDa
Observed Molecular Weight 49-50 kDa
GenBank Accession NumberBC064623
Gene Symbol POFUT2
Gene ID (NCBI) 23275
RRIDAB_1959662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POFUT2 antibody 17764-1-APDownload protocol
IHC protocol for POFUT2 antibody 17764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Mol Med

A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder

Authors - Rene G Feichtinger
humanIHC

Cell Death Dis

LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.

Authors - S Liu

Schizophr Res

Altered fucosyltransferase expression in the superior temporal gyrus of elderly patients with schizophrenia.

Authors - Toni M Mueller
humanWB

Dev Biol

Genetic and biochemical evidence that gastrulation defects in Pofut2 mutants result from defects in ADAMTS9 secretion.

Authors - Brian A Benz

Methods Mol Biol

Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays.

Authors - Steven John Berardinelli
humanWB

J Cancer Res Clin Oncol

Emerging glyco-risk prediction model to forecast response to immune checkpoint inhibitors in colorectal cancer

Authors - Peishan Qiu
  • KD Validated