POLR3A Polyclonal antibody

POLR3A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17530-1-AP

Synonyms

hRPC155, POLR3A, RNA polymerase III subunit C1, RPC1, RPC155



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17530-1-AP targets POLR3A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR3A fusion protein Ag11536
Full Name polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
Calculated Molecular Weight 1390 aa, 156 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC041089
Gene Symbol POLR3A
Gene ID (NCBI) 11128
RRIDAB_2167983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLR3A antibody 17530-1-APDownload protocol
IHC protocol for POLR3A antibody 17530-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,IP

Cell Rep

Subcellular localization shapes the fate of RNA polymerase III

Authors - Kai Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rebecca (Verified Customer) (10-28-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: iPSC-CM
POLR3A Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:17530-1-AP)
FH

Krishnendu (Verified Customer) (02-27-2020)

Didn't see any bands even after using different concentrations of lysate.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293T