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POLR3F Polyclonal antibody

POLR3F Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 16044-1-AP

Synonyms

POLR3F, RNA polymerase III subunit C6, RPC39, RPC6

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using POLR3F Polyclonal antibody (16044-1-AP)

    WB analysis of human brain using 16044-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16044-1-AP (POLR3F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLR3F Polyclonal antibody (16044-1-AP)

    WB analysis of HeLa using 16044-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16044-1-AP (POLR3F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using POLR3F Polyclonal antibody (16044-1-AP)

    WB analysis of BxPC-3 using 16044-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16044-1-AP (POLR3F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR3F Polyclonal antibody (16044-1-AP)

    IF Staining of HepG2 using 16044-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16044-1-AP (POLR3F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, BxPC-3 cells, HeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16044-1-AP targets POLR3F in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR3F fusion protein Ag8935
Full Name polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
Calculated Molecular Weight 316 aa, 36 kDa
Observed Molecular Weight 36-39 kDa
GenBank Accession NumberBC012588
Gene Symbol POLR3F
Gene ID (NCBI) 10621
RRIDAB_2165707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLR3F antibody 16044-1-APDownload protocol
IF protocol for POLR3F antibody 16044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols