Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HCT 116 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15645-1-AP targets POM121 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8177 Product name: Recombinant human POM121 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 635-984 aa of BC008794 Sequence: SQSLHTAVPTATSSSAADFSGFGSTLATSAPATSSQPTLTFSNTSTPTFNIPFGSSAKSPLPSYPGANPQPAFGAAEGQPPGAAKPALAPSFGSSFTFGNSAAPAAAPTPAPPSMIKVVPAYVPTPIHPIFGGATHSAFGLKATASAFGAPASSQPAFGGSTAVFFGAATSSGFGATTQTASSGSSSSVFGSTTPSPFTFGGSAAPAGSGSFGINVATPGSSTTTGAFSFGAGQSGSTATSTPFAGGLGQNALGTTGQSTPFAFNVSSTTESKPVFGGTATPTFGLNTPAPGVGTSGSSLSFGASSAPAQGFVGVAPFGSAALSFSIGAGSKTPGARQRLQARRQHTRKK Predict reactive species |
| Full Name | POM121 membrane glycoprotein (rat) |
| Calculated Molecular Weight | 984 aa, 99 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC008794 |
| Gene Symbol | POM121 |
| Gene ID (NCBI) | 9883 |
| RRID | AB_2878160 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for POM121 antibody 15645-1-AP | Download protocol |
| IHC protocol for POM121 antibody 15645-1-AP | Download protocol |
| WB protocol for POM121 antibody 15645-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The POM121 polyclonal antibody (Cat# 15645-1-AP) has 12 citations published in journals including Nature, Nature Neuroscience, Developmental Cell, PLoS Pathogens, Journal of Molecular Biology, Tissue & Cell, Cytotechnology, Cancer Biomarkers, eLife, Journal of Cell Science, Methods in Molecular Biology, and bioRxiv. Research fields span nuclear envelope architecture, nucleocytoplasmic transport in ALS, nuclear pore complex function, viral infection (cardiovirus, HIV-1), lung cancer (proliferation, metastasis, chemoresistance), and cellular senescence.
| Species | Application | Title |
|---|---|---|
Nat Neurosci Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway. | ||
PLoS Pathog Cardiovirus leader proteins retarget RSK kinases toward alternative substrates to perturb nucleocytoplasmic traffic | ||
J Mol Biol Interactions between FUS and the C-terminal domain of Nup62 are sufficient for their co-phase separation into amorphous assemblies | ||
Tissue Cell Genetic haploinsufficiency of POM121 exacerbates ischemia reperfusion injury induced acute kidney injury by promoting p53/p21-mediated cellular senescence. |
Reviews
What customers say
Users report positive results with this antibody for Western Blot and Immunofluorescence. One reviewer (5/5) used it at 1:5000 in WB on Hela and Vero cell lysates with 5% milk blocking, noting strong performance. Another (4/5) applied it to human neurons at 1:400 for IF and 1:2000 for WB. Overall, the antibody is well-received for its sensitivity and reliability across cell types and applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
bing (Verified Customer) (08-06-2019) | Works great in Western Blot, I use it for Hela and Vero cells lysate, go dilution 1:5000 in 5% milk.
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Yen-Chen (Verified Customer) (12-03-2018) |
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