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  • Featured Product
  • KD/KO Validated

POMP Polyclonal antibody

POMP Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15046-1-AP

Synonyms

C13orf12, HSPC014, HSPC036, hUMP1, PNAS 110, POMP, proteasome maturation protein, Proteassemblin, Protein UMP1 homolog, UMP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using POMP Polyclonal antibody (15046-1-AP)

    WB analysis of HeLa using 15046-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15046-1-AP (POMP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using POMP Polyclonal antibody (15046-1-AP)

    WB analysis of HepG2 using 15046-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15046-1-AP (POMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using POMP Polyclonal antibody (15046-1-AP)

    WB analysis of L02 using 15046-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15046-1-AP (POMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using POMP Polyclonal antibody (15046-1-AP)

    IHC staining of human liver using 15046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using POMP Polyclonal antibody (15046-1-AP)

    IHC staining of human liver using 15046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skin tissue using POMP Polyclonal antibody (15046-1-AP)

    IHC staining of human skin using 15046-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skin tissue using POMP Polyclonal antibody (15046-1-AP)

    IHC staining of human skin using 15046-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POMP Polyclonal antibody (15046-1-AP)

    IF Staining of HepG2 using 15046-1-AP

    Immunofluorescent analysis of HepG2 cells, using POMP antibody 15046-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POMP Polyclonal antibody (15046-1-AP)

    IF Staining of HeLa using 15046-1-AP

    Immunofluorescent analysis of Hela cells, using POMP antibody 15046-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Flow cytometry (FC) experiment of HepG2 cells using POMP Polyclonal antibody (15046-1-AP)

    FC experiment of HepG2 using 15046-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug POMP antibody (15046-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IHC detected inhuman liver tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15046-1-AP targets POMP in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POMP fusion protein Ag6987
Full Name proteasome maturation protein
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC003390
Gene Symbol POMP
Gene ID (NCBI) 51371
RRIDAB_10858321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POMP antibody 15046-1-APDownload protocol
IHC protocol for POMP antibody 15046-1-APDownload protocol
IF protocol for POMP antibody 15046-1-APDownload protocol
FC protocol for POMP antibody 15046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Dermatol Sci

The proteasome maturation protein POMP increases proteasome assembly and activity in psoriatic lesional skin.

Authors - Barbara A Zieba
  • KD Validated
View Article
mouseWB

JCI Insight

Blockade of the CXCR3/CXCL10 axis ameliorates inflammation caused by immunoproteasome dysfunction.

Authors - Yuki Sasaki
View Article