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POP7 Polyclonal antibody

POP7 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 14964-1-AP

Synonyms

0610037N12Rik, hPOP7, POP7, RNaseP protein p20, RPP2, RPP20

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using POP7 Polyclonal antibody (14964-1-AP)

    WB analysis of HeLa using 14964-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14964-1-AP (POP7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POP7 Polyclonal antibody (14964-1-AP)

    WB analysis of HeLa using 14964-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14964-1-AP (POP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using POP7 Polyclonal antibody (14964-1-AP)

    IP experiment of HeLa using 14964-1-AP

    IP result of anti-POP7 (IP:14964-1-AP, 4ug; Detection:14964-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POP7 Polyclonal antibody (14964-1-AP)

    IF Staining of HeLa using 14964-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14964-1-AP (POP7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14964-1-AP targets POP7 in WB, IP, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POP7 fusion protein Ag6827
Full Name processing of precursor 7, ribonuclease P/MRP subunit (S. cerevisiae)
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC001430
Gene Symbol POP7
Gene ID (NCBI) 10248
RRIDAB_2166488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POP7 antibody 14964-1-APDownload protocol
IF protocol for POP7 antibody 14964-1-APDownload protocol
IP protocol for POP7 antibody 14964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.

Authors - Jian Zhang
View Article