POU4F3 Polyclonal antibody
POU4F3 Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21509-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Neuro-2a cells, HL-60 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
Product Information
The immunogen of 21509-1-AP is POU4F3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | POU4F3 fusion protein Ag16004 |
| Full Name | POU class 4 homeobox 3 |
| Calculated Molecular Weight | 338 aa, 37 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC104923 |
| Gene Symbol | POU4F3 |
| Gene ID (NCBI) | 5459 |
| RRID | AB_2878872 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POU4F3 antibody 21509-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. | ||
Adv Sci (Weinh) Injectable, Antioxidative, and Tissue-Adhesive Nanocomposite Hydrogel as a Potential Treatment for Inner Retina Injuries | ||
Front Cell Dev Biol In vivo Regeneration of Ganglion Cells for Vision Restoration in Mammalian Retinas. | ||
Sci Rep 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively. | ||
Neurochem Int Human primary retinal cells as an in-vitro model for investigating defective signalling caused by OPTN mutants associated with glaucoma. | ||
Hear Res Sox2 is required in supporting cells for normal levels of vestibular hair cell regeneration in adult mice |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH AMRITA (Verified Customer) (02-15-2021) | The antibody requires a prior antigen retrieval step for 15min in 80 deg centigrade
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