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WB analysis of HEK-293 using 16662-1-AP
WB result of PPA2 antibody (16662-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPA2 transfected HEK-293 cells.
WB analysis of mouse heart using 16662-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 16662-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16662-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 16662-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16662-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16662-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16662-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16662-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16662-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

PPA2 Polyclonal antibody

PPA2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16662-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:3.6.1.1, HSPC124, PPase 2, Pyrophosphatase SID6 306, Pyrophosphatase SID6-306

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue, mouse kidney tissue, HEK-293 cells, mouse liver tissue, rat heart tissue
Positive IHC detected in	human liver cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below

Product Information

16662-1-AP targets PPA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10138 Product name: Recombinant human PPA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC057219 Sequence: MSALLRLLRTGAPAAACLRLGTSAGTGSRRAMALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKVNSKEENGIPMKKARNDEYENLFNMIVEIPRWTNAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIWNYGTLPQILSCGEVIHVKILGILALIDEGETDWKLIAINANDPEASKFHDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCTNVQISDSPFRCTQEEARSLVESVSSSPNKESNEEEQVWHFLGK Predict reactive species
Full Name	pyrophosphatase (inorganic) 2
Calculated Molecular Weight	305aa,35 kDa; 334aa,38 kDa
Observed Molecular Weight	32-40 kDa
GenBank Accession Number	BC057219
Gene Symbol	PPA2
Gene ID (NCBI)	27068
RRID	AB_1851549
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H2U2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

Protocols

Product Specific Protocols
IHC protocol for PPA2 antibody 16662-1-AP	Download protocol
WB protocol for PPA2 antibody 16662-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Front Immunol Multi-omics pan-cancer analysis reveals the prognostic values and immunological functions of PPA2, with a spotlight on breast cancer Authors - Jia-Ning Zhang View Article