Tested Applications
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 19 publications below |
| CoIP | See 1 publications below |
Product Information
22378-1-AP targets PPARGC1B in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, zebrafish, goat, fish, megalobrama amblycephala |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17566 Product name: Recombinant human PPARGC1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC132971 Sequence: MAGNDCGALLDEELSSFFLNYLADTQGGGSGEEQLYADFPELDLSQLDASDFDSATCFGELQWCPENSETEPNQYSPDDSELFQIDSENEALLAELTKTLDDIPEDDVGLAAFPALDGGDALSCTSASPAPSSAPPSPAPEKPSAPAPEVDELSLLQKLLLATSYPTSSSDTQKEGTAWRQAGLRSKSQRPCVKADSTQDKKAPMMQSQSRSCTELHKHLTSAQCCLQDRGLQPPCLQSPRLPAKEDKEPGEDCPSPQPAPASPRDSLALGRADPGAPVSQEDMQAMVQLIRYMHTYCLPQRKLPPQTPEPLPKACSNPSQQVRSRPWSRHHSKASWAEFSILRELLAQD Predict reactive species |
| Full Name | peroxisome proliferator-activated receptor gamma, coactivator 1 beta |
| Calculated Molecular Weight | 1023 aa, 113 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC132971 |
| Gene Symbol | PPARGC1B |
| Gene ID (NCBI) | 133522 |
| RRID | AB_2879093 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86YN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPARGC1B (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Beta) is a transcriptional coactivator that plays a key role in the regulation of cellular energy metabolism, particularly in tissues with high metabolic demands such as the heart, skeletal muscle, liver, and brain. Encoded by the PPARGC1B gene in humans, it interacts with a variety of nuclear receptors and transcription factors, including PPARs, ERRs, and NRF1, to promote the expression of genes involved in mitochondrial biogenesis, oxidative phosphorylation, fatty acid oxidation, and glucose homeostasis. Unlike its close paralog PGC-1α, PGC-1β is less responsive to physiological stimuli like cold exposure or exercise but is more constitutively expressed and tightly linked to lipid metabolism and reactive oxygen species (ROS) detoxification.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for PPARGC1B antibody 22378-1-AP | Download protocol |
| WB protocol for PPARGC1B antibody 22378-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation | ||
Br J Pharmacol Andrographolide prevents bone loss via targeting ERRalpha-regulated metabolic adaption of osteoclastogenesis. | ||
EMBO Rep Mitophagy receptor FUNDC1 is regulated by PGC-1α/NRF1 to fine tune mitochondrial homeostasis.
| ||
J Anim Sci Biotechnol miR-25 modulates triacylglycerol and lipid accumulation in goat mammary epithelial cells by repressing PGC-1beta. | ||
Nutrients Novel Choline-Deficient and 0.1%-Methionine-Added High-Fat Diet Induces Burned-Out Metabolic-Dysfunction-Associated Steatohepatitis with Inflammation by Rapid Immune Cell Infiltration on Male Mice | ||
FASEB J Hypolipidemic effect of oleanolic acid is mediated by the miR-98-5p/PGC-1β axis in high-fat diet-induced hyperlipidemic mice.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Mounika (Verified Customer) (12-25-2025) | Antibodies are working good, visibility is good!
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