Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Calu-1 cells, LNCaP cells |
| Positive IHC detected in | human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18001-1-AP targets PPCS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12491 Product name: Recombinant human PPCS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-311 aa of BC106064 Sequence: MAEMDPVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEAEENALPGFAEALRSYQEAAAAGTFLAVEFTTLADYLHLLQAAAQALNPLGPSAMFYLAAAVSDFYVPVSEMPEHKIQSSGGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVINRARKALEIYQHQVVVANILESRQSFVFIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQSRHTAFIGDRN Predict reactive species |
| Full Name | phosphopantothenoylcysteine synthetase |
| Calculated Molecular Weight | 311 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC106064 |
| Gene Symbol | PPCS |
| Gene ID (NCBI) | 79717 |
| RRID | AB_10548725 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPCS(Phosphopantothenate--cysteine ligase) is also named as COAB and belongs to the PPC synthetase family. It catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. It can be exsit as a homodimer(PMID:12906824).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPCS antibody 18001-1-AP | Download protocol |
| WB protocol for PPCS antibody 18001-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The polyclonal antibody against PPCS (Phosphopantothenate–cysteine ligase), catalog number 18001-1-AP, has a total of 2 citations. These appear in International Journal of Molecular Sciences and FEBS Journal. The cited research fields include coenzyme A biosynthetic complex assembly in mammalian cells and ferroptosis regulation via iron-starvation response suppression. Both studies used human samples, with applications in immunofluorescence (IF) and Western blot (WB).
| Species | Application | Title |
|---|---|---|
FEBS J Coenzyme A mitigates cystine-deprivation-induced ferroptosis by suppressing the iron-starvation response. |
Reviews
What customers say
One user (Jovana Bakovic) rated this antibody 4/5 for Western Blot. She reported it worked well to detect endogenous PPCS protein in HEK293 cells at a 1:1000 dilution with a 2-hour incubation at room temperature. Overall, the single review reflects positive performance for WB applications in mammalian cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jovana (Verified Customer) (09-17-2020) | The antibody worked well to detect endogenous PPCS protein on WB in HEK293 cells. Dilution used was 1:1000 and incubation was 2h at RT.
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