Tested Applications
| Positive WB detected in | BxPC-3 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15357-1-AP targets Cyclophilin E in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7603 Product name: Recombinant human PPIE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC004898 Sequence: MATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDDWLKKFSGKTLEENKEEEGSEPPKAETQEGEPIAKKARSNPQVYMDIKIGNKPAGRIQMLLRSDVVPMTAENFRCLCTHEKGFGFKGSSFHRIIPQFMCQGGDFTNHNGTGGKSIYGKKFDDENFILKHTGPGLLSMANSGPNTNGSQFFLTCDKTDWLDGKHVVFGEVTEGLDVLRQIEAQGSKDGKPKQKVIIADCGEYV Predict reactive species |
| Full Name | peptidylprolyl isomerase E (cyclophilin E) |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33-38 kDa |
| GenBank Accession Number | BC004898 |
| Gene Symbol | Cyclophilin E |
| Gene ID (NCBI) | 10450 |
| RRID | AB_2878131 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNP9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cyclophilin E (CypE), also known as Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type PPIase family of PPIase E subfamily (PMID: 15998457). Cyclophilin E negatively regulates influenza virus replication and transcription occurs by impairing the formation of the vRNP (PMID: 21887220). Additionally, Cyclophilin E plays an important role in osteoblast differentiation as a positive regulator by increasing the transcriptional activity of Runx2 (PMID: 37947627).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cyclophilin E antibody 15357-1-AP | Download protocol |
| IHC protocol for Cyclophilin E antibody 15357-1-AP | Download protocol |
| WB protocol for Cyclophilin E antibody 15357-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aleksandr (Verified Customer) (07-19-2025) | Overall antibody recognize the band of the expected size, but there are several non-specific bands
 |
Moritz (Verified Customer) (09-12-2024) | Several bands were observable in WB, also the expected size of PPIE, although it was not the strongest.
 |
Tsimafei (Verified Customer) (08-12-2024) | Multiple bands are present, however the one corresponding to Cyclophilin E size (~33 kDa) is clearly visible
 |
