PPIF-Specific Antibody
Rabbit Polyclonal
Catalog number: 18466-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | human heart tissue, HeLa cells, HepG2 cells |
| Positive IHC detected in | human heart |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
human heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP(PPIF-Specific Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human heart using 18466-1-AP(PPIF-Specific Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart using 18466-1-AP(PPIF-Specific Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using PPIF antibody 18466-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | PPIF-Specific fusion protein ag13151 | Full name | peptidylprolyl isomerase F |
|
Calculated molecular weight |
22kd |
Observed molecular
weight |
22kd |
|
GenBank accession number |
BC005020 | Gene ID (NCBI) | 10105 |
| Gene symbol | PPIF | Synonyms | CYP3; Cyp-D; FLJ90798; MGC117207 |
Background
PPIF, also named as CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is special to PPIF |
