Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13193-1-AP targets PPM1B in WB, IHC, IF/ICC, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3871 Product name: Recombinant human PPM1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-192 aa of BC012002 Sequence: MSIVLVCFSNAPKVSDEAVKKDSELDKHLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSNEDAGTKMSGEKI Predict reactive species |
| Full Name | protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform |
| Calculated Molecular Weight | 479 aa, 53 kDa |
| Observed Molecular Weight | 36, 53 kDa |
| GenBank Accession Number | BC012002 |
| Gene Symbol | PPM1B |
| Gene ID (NCBI) | 5495 |
| RRID | AB_10644118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75688 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPM1B is a member of the metal-dependent serine/threonine protein phosphatase (PPM) family with important regulatory functions in cellular signalling pathways(PMID: 18066953). PPM1B is also an important negative regulator of several stress signalling pathways and has been shown to negatively regulate the stress-activated p38 and JNK pathways by directly dephosphorylating upstream signalling proteins(PMID: 9824284). Moreover, PPM1B has also been shown to prevent cell death by negatively regulating necrotic cell death in a RIP3 dephosphorylation-dependent manner.(PMID: 25751141) PPM1B can be detected as the WM of 36-55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPM1B antibody 13193-1-AP | Download protocol |
| IHC protocol for PPM1B antibody 13193-1-AP | Download protocol |
| WB protocol for PPM1B antibody 13193-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PPM1B antibody (Cat# 13193-1-AP) has 8 total citations across journals including Signal Transduction and Targeted Therapy, Nature Communications, Cell Reports, Frontiers in Pharmacology, Biochimica et Biophysica Acta Molecular Cell Research, Experimental Cell Research, and bioRxiv. Research fields span colorectal and breast cancer, obesity and hepatic steatosis, cardiomyopathy, spinal cord injury, phosphatase structural biology, and cell cycle regulation. Applications include WB, IP, IF, and cell treatment in human and mouse models.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting EHMT2 overcomes 5-fluorouracil resistance in colorectal cancer by modulating cell cycle and apoptosis. | ||
Cell Rep Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis | ||
Front Pharmacol Initial Characterization of Stressed Transgenic Mice With Cardiomyocyte-Specific Overexpression of Protein Phosphatase 2C. | ||
Front Pharmacol Icariin promotes functional recovery in rats after spinal cord injury by inhibiting YAP and regulating PPM1B ubiquitination to inhibiting the activation of reactive astrocytes | ||
Biochim Biophys Acta Mol Cell Res TXLNA enhances TBK1 phosphorylation by suppressing PPM1B recruitment |
