Tested Applications
| Positive WB detected in | MCF-7 cells, mouse lung tissue, rat testis tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26532-1-AP targets PPM1D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, Rat samples.
| Tested Reactivity | human, mouse, Rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24751 Product name: Recombinant human PPM1D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 254-423 aa of BC060877 Sequence: NGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHTLDPQKHKYIILGSDGLWNMIPQQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVKSLE Predict reactive species |
| Full Name | protein phosphatase 1D magnesium-dependent, delta isoform |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC060877 |
| Gene Symbol | PPM1D |
| Gene ID (NCBI) | 8493 |
| RRID | AB_2880544 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15297 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPM1D antibody 26532-1-AP | Download protocol |
| WB protocol for PPM1D antibody 26532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPM1D Polyclonal antibody (Cat# 26532-1-AP) has 1 total citation. It was cited in Radiation Research (2023), in a study investigating temporal dynamic regulation of autophagy and senescence induction in response to radiation exposure. The research field is radiation biology, specifically focusing on autophagy and cellular senescence mechanisms. The antibody was used for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Radiat Res Temporal Dynamic Regulation of Autophagy and Senescence Induction in Response to Radiation Exposure |
