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WB analysis of MCF-7 using 17020-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 17020-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17020-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17020-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 17020-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17020-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 17020-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 17020-1-AP
IP result of anti-PPM1F (IP:17020-1-AP, 4ug; Detection:17020-1-AP 1:700) with MCF-7 cells lysate 2800ug.
IHC staining of human colon cancer using 17020-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 17020-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 17020-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PPM1F antibody (17020-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

PPM1F Polyclonal antibody

Name: PPM1F Polyclonal antibody
Brand: Proteintech
SKU: 17020-1-AP

PPM1F Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17020-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM kinase phosphatase, CaM-kinase phosphatase, CaMKPase, EC:3.1.3.16

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, L02 cells, HepG2 cells, human liver tissue, K-562 cells, HeLa cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human ovary cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below

Product Information

17020-1-AP targets PPM1F in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10186 Product name: Recombinant human PPM1F protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 104-453 aa of BC071989 Sequence: EEEEDDDEEKAPVTLLDAQSLAQSFFNRLWEVAGQWQKQVPLAARASQRQWLVSIHAIRNTRRKMEDRHVSLPSFNQLFGLSDPVNRAYFAVFDGHGGVDAARYAAVHVHTNAARQPELPTDPEGALREAFRRTDQMFLRKAKRERLQSGTTGVCALIAGATLHVAWLGDSQVILVQQGQVVKLMEPHRPERQDEKARIEALGGFVSHMDCWRVNGTLAVSRAIGDVFQKPYVSGEADAASRALTGSEDYLLLACDGFFDVVPHQEVVGLVQSHLTRQQGSGLRVAEELVAAARERGSHDNITVMVVFLRDPQELLEGGNQGEGDPQAEGRRQDLPSSLPEPETQAPPRS Predict reactive species
Full Name	protein phosphatase 1F (PP2C domain containing)
Calculated Molecular Weight	453 aa, 50 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC071989
Gene Symbol	PPM1F
Gene ID (NCBI)	9647
RRID	AB_2284338
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49593
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

Protocols

Product Specific Protocols
IF protocol for PPM1F antibody 17020-1-AP	Download protocol
IHC protocol for PPM1F antibody 17020-1-AP	Download protocol
IP protocol for PPM1F antibody 17020-1-AP	Download protocol
WB protocol for PPM1F antibody 17020-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Cell Biol PPM1F controls integrin activity via a conserved phospho-switch. Authors - Tanja M Grimm KO Validated View Article
human	WB	Clin Transl Oncol PPM1F regulates ovarian cancer progression by affecting the dephosphorylation of ITGB1 Authors - Yahui Leng View Article