Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18203-1-AP targets PPM1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12775 Product name: Recombinant human PPM1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-233 aa of BC104885 Sequence: MPAFSTTAAEYVKSRLPEALKQHLQDYEKDKENSVLSYQTILEQQILSIDREMLEKLTVSYDEAGTTCLIALLSDKDLTVANVGDSRGVLCDKDGNAIPLSHDHKPYQLKERKRIKRAGGFISFNGSWRVQGILAMSRSLGDYPLKNLNVVIPDPDILTFDLDKLQPEFMILASDGLWDAFSNEEAVRFIKERLDEPHFGAKSIVLQSFYRGCPDNITVMVVKFRNSSKTEEQ Predict reactive species |
| Full Name | protein phosphatase 1 (formerly 2C)-like |
| Calculated Molecular Weight | 26 kDa, 41 kDa |
| Observed Molecular Weight | 41-45 kDa |
| GenBank Accession Number | BC104885 |
| Gene Symbol | PPM1L |
| Gene ID (NCBI) | 151742 |
| RRID | AB_10859821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5SGD2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPM1L(Protein phosphatase 1L) is also named as PP2CE.It belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. It has 4 isoforms produced by alternative splicing.This antibody is specific to PPM1L.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPM1L antibody 18203-1-AP | Download protocol |
| WB protocol for PPM1L antibody 18203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPM1L Polyclonal antibody (Cat# 18203-1-AP) has 1 total citation. The cited publication appeared in Cell Death & Disease (Impact Factor 12.2), published in September 2022. The study focused on direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration, placing it in the fields of stem cell biology and ophthalmology/regenerative medicine. The antibody was used in Western Blot (WB) for human samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration |
