Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse brain tissue, fetal human brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human heart tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55148-1-AP targets PPP3CB-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | NM_021132 |
| Gene Symbol | PPP3CB |
| Gene ID (NCBI) | 5532 |
| RRID | AB_10859260 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16298 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CB.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP3CB-specific antibody 55148-1-AP | Download protocol |
| IHC protocol for PPP3CB-specific antibody 55148-1-AP | Download protocol |
| IP protocol for PPP3CB-specific antibody 55148-1-AP | Download protocol |
| WB protocol for PPP3CB-specific antibody 55148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody PPP3CB (catalog no. 55148-1-AP) has 6 total citations. These appear in Cancer Communications, Journal of Clinical Investigation, Molecular Psychiatry, Acta Pharmacologica Sinica, Journal of Agricultural and Food Chemistry, and Journal of Molecular Medicine. Research fields span hepatocellular carcinoma, podocyte biology and nephrotoxicity, autism spectrum disorder, cerebral ischemia/neuroprotection, and calcium/calcineurin signaling pathways.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression | ||
J Clin Invest MicroRNA-30 family members regulate calcium/calcineurin signaling in podocytes. | ||
Mol Psychiatry A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling. | ||
Acta Pharmacol Sin MCOLN1-mediated PPP3CB activation alleviates neuronal damage by promoting TFEB-dependent autophagic flux in permanent cerebral ischemia. | ||
J Agric Food Chem Astragaloside IV Mitigates Tacrolimus-Induced Chronic Nephrotoxicity by Regulating the mTOR-TFEB-GADD45α Pathway. | ||
J Mol Med (Berl) Angiotensin II induces calcium/calcineurin signaling and podocyte injury by downregulating microRNA-30 family members. |
Reviews
What customers say
One user rated this antibody 5 out of 5 stars. They used it at a 1:1000 dilution for Western Blot on a CRISPR knock-in HEK293 cell line. While they noted a couple of non-specific bands appeared, the antibody still provided the specific signal they needed for their application. Overall, the reviewer found it effective for their purpose despite minor background issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
JIN-FENG (Verified Customer) (11-24-2025) | I used the ab for CRIPSR KI cell line generation. Although it showed a couple of non-specific bands, it still provided the signal I am looking for.
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