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PQBP1 Polyclonal antibody

PQBP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16264-1-AP

Synonyms

MRX55, MRXS3, MRXS8, PQBP 1, PQBP1, RENS1, SHS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PQBP1 Polyclonal antibody (16264-1-AP)

    WB analysis using 16264-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PQBP1 Polyclonal antibody (16264-1-AP)

    WB analysis of rat brain using 16264-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PQBP1 Polyclonal antibody (16264-1-AP)

    WB analysis of mouse skeletal muscle using 16264-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PQBP1 Polyclonal antibody (16264-1-AP)

    WB analysis of mouse brain using 16264-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PQBP1 Polyclonal antibody (16264-1-AP)

    WB analysis of HeLa using 16264-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PQBP1 Polyclonal antibody (16264-1-AP)

    WB analysis of HeLa using 16264-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PQBP1 Polyclonal antibody (16264-1-AP)

    IP experiment of mouse brain using 16264-1-AP

    IP result of anti-PQBP1 (IP:16264-1-AP, 4ug; Detection:16264-1-AP 1:500) with mouse brain tissue lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PQBP1 Polyclonal antibody (16264-1-AP)

    IHC staining of human breast cancer using 16264-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PQBP1 Polyclonal antibody (16264-1-AP)

    IHC staining of human colon using 16264-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using PQBP1 Polyclonal antibody (16264-1-AP)

    IHC staining of rat colon using 16264-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PQBP1 Polyclonal antibody (16264-1-AP)

    IF Staining of HeLa using 16264-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16264-1-AP (PQBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16264-1-AP targets PQBP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PQBP1 fusion protein Ag9329
Full Name polyglutamine binding protein 1
Calculated Molecular Weight 265 aa, 30 kDa
Observed Molecular Weight30-32 kDa
GenBank Accession NumberBC012358
Gene Symbol PQBP1
Gene ID (NCBI) 10084
RRIDAB_10792928
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PQBP1 (Polyglutamine-binding protein 1) is also named as NPW38 and JM26. PQBP1 senses extrinsic tau 3R/4R proteins by direct interaction and triggers an innate immune response by activating a cyclic GMP-AMP synthase (cGAS)-Stimulator of interferon genes (STING) pathway (PMID: 34782623).  PQBP1 is abundantly expressed in the central nervous system during development. PQBP1 is a component of the precatalytic spliceosome (B complex) that is involved in splicing. PQBP1 was predominantly localized in the cell nucleus. PQBP1 is overexpressed in various cancer types including breast cancer, colon cancer, and glioblastoma (PMID: 38342602).

Protocols

Product Specific Protocols
WB protocol for PQBP1 antibody 16264-1-APDownload protocol
IHC protocol for PQBP1 antibody 16264-1-APDownload protocol
IF protocol for PQBP1 antibody 16264-1-APDownload protocol
IP protocol for PQBP1 antibody 16264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IP, IF

Mol Cell

PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.

Authors - Yuqian Shen
View Article
humanIHC

Acta Neuropathol

Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

Authors - Neumann Manuela M
View Article
humanWB

mBio

Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response.

Authors - Hongming Ma
View Article