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WB analysis of L02 using 11932-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 11932-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11932-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 11932-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 11932-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 11932-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11932-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse small intestine using 11932-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of mouse small intestine using 11932-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

PRAP1 Polyclonal antibody

Name: PRAP1 Polyclonal antibody
Brand: Proteintech
SKU: 11932-1-AP

PRAP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11932-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

PRAP1, PRO1195, proline rich acidic protein 1, RP11 122K13.6, UPA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	L02 cells, mouse pancreas tissue
Positive IHC detected in	human liver tissue, human kidney tissue, mouse small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

11932-1-AP targets PRAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2526 Product name: Recombinant human PRAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC029447 Sequence: RLLLVTSLVVVLLWEAGAVPAPKVPIKMQVKHWPSEQDPENRAWGARVVEPPEKDDQLVVLFPVQKPKLLTTEEKPRGQGRGPILPGTKAWMETEDTLGRVLSPEPDHDSLYHPPPEEDQGEERPRLWVMPNHQVLLGPEEDQDHIYHPQ Predict reactive species
Full Name	proline-rich acidic protein 1
Calculated Molecular Weight	151 aa, 17 kDa
Observed Molecular Weight	16-20 kDa
GenBank Accession Number	BC029447
Gene Symbol	PRAP1
Gene ID (NCBI)	118471
RRID	AB_10951856
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96NZ9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.

Protocols

Product Specific Protocols
IHC protocol for PRAP1 antibody 11932-1-AP	Download protocol
WB protocol for PRAP1 antibody 11932-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC,WB	Adv Sci (Weinh) Adipocyte-Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer Authors - Qiumo Zhang KD Validated View Article
human,mouse	WB,IF	Nat Commun Single-cell RNA sequencing reveals placental response under environmental stress Authors - Eric Van Buren View Article
human	WB	Research (Wash D C) Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell-Cell Fusion and Immune Activation Authors - Yao Wang View Article
human	IF	Elife scRNA-seq and scATAC-seq reveal that Sertoli cell mediates spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia Authors - Shimin Wang View Article