• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PRDM5 Polyclonal antibody

PRDM5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14126-1-AP

Synonyms

PFM2, PR domain containing 5, PR domain containing protein 5, PRDM5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse ovary tissue using PRDM5 Polyclonal antibody (14126-1-AP)

    WB analysis of mouse ovary using 14126-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14126-1-AP (PRDM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using PRDM5 Polyclonal antibody (14126-1-AP)

    WB analysis of mouse colon using 14126-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 14126-1-AP (PRDM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRDM5 Polyclonal antibody (14126-1-AP)

    IHC staining of human colon cancer using 14126-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14126-1-AP (PRDM5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRDM5 Polyclonal antibody (14126-1-AP)

    IHC staining of human colon cancer using 14126-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14126-1-AP (PRDM5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse ovary tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14126-1-AP targets PRDM5 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDM5 fusion protein Ag5277
Full Name PR domain containing 5
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC066942
Gene Symbol PRDM5
Gene ID (NCBI) 11107
RRIDAB_2878019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRDM5 antibody 14126-1-APDownload protocol
IHC protocol for PRDM5 antibody 14126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols