PRIM2 Polyclonal antibody

PRIM2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 11788-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PRIM2A, p58

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIF/ICC

WB analysis using 11788-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11788-1-AP (PRIM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A375 using 11788-1-AP
Immunofluorescent analysis of (4% PFA) fixed A375 cells using 11788-1-AP (PRIM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	A375 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IF/ICC detected in	A375 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

11788-1-AP targets PRIM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2370 Product name: Recombinant human PRIM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-158 aa of BC017833 Sequence: MEFSGRKWRKLRLAGDQRNASYPHCLQFYLQPPSENISLIEFENLAIDRVKLLKSVENLGVSYVKGTEQYQSKLESELRKLKFSYRENLEDEYEPRRRDHISHFILRLAYCQSEELRRWFIQQEMDLLRFRFSILPKDKIQDFLKDSQLQFEAVSIFL Predict reactive species
Full Name	primase, DNA, polypeptide 2 (58kDa)
Calculated Molecular Weight	58 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	BC017833
Gene Symbol	PRIM2
Gene ID (NCBI)	5558
RRID	AB_2169380
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49643
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for PRIM2 antibody 11788-1-AP	Download protocol
WB protocol for PRIM2 antibody 11788-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Med Oncol PRIM2 promotes proliferation and metastasis of pancreatic ductal adenocarcinoma through interactions with FAM111B Authors - Jingyang Yin KD Validated View Article
human	WB,IHC	J Exp Clin Cancer Res LARS promotes osteosarcoma proliferation through leucine-dependent PRIM2 translation and DNA replication activation. Authors - Guanan Bai KD Validated View Article
		Mol Cell The TIM22 carrier translocase supports cell proliferation by facilitating mitochondrial iron uptake for Fe-S biogenesis. Authors - Shuai Liu View Article
human	WB	Biochim Biophys Acta Gen Subj Primase subunit 2 regulates the proliferation and apoptosis of gastric cancer cells via MCM7/CDK4 and Bax/Bcl-2 pathways. Authors - Bingbing Liu KD Validated View Article