PRKACB Antibody
Rabbit Polyclonal
Catalog number: 12232-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HT-1080 cells, A549 cells, MCF7 cells, mouse brain tissue, PC-3 cells |
| Positive IHC detected in | human medulloblastoma |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HT-1080 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP(PRKACB Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human medulloblastoma using 12232-1-AP(PRKACB Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using PRKACB antibody 12232-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | PRKACB fusion protein ag2870 | Full name | protein kinase, cAMP-dependent, catalytic, beta |
|
Calculated molecular weight |
41kd;46kd |
Observed molecular
weight |
46-55kd |
|
GenBank accession number |
BC016285 | Gene ID (NCBI) | 5567 |
| Gene symbol | PRKACB | Synonyms | DKFZp781I2452; MGC41879; MGC9320; PKACB |
Background
PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. This antibody is specific to PRKACB. |
