Filter: AllWBIHC

WB analysis of mouse brain using 17991-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17991-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 17991-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRKAR1B Polyclonal antibody

Name: PRKAR1B Polyclonal antibody
Brand: Proteintech
SKU: 17991-1-AP

PRKAR1B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17991-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

cAMP-dependent protein kinase type I-beta regulatory subunit, PRKAR1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, Transfected HEK-293 cells, HeLa cells
Positive IHC detected in	human breast cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17991-1-AP targets PRKAR1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12426 Product name: Recombinant human PRKAR1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-381 aa of BC026734 Sequence: MASPPACPSEEDESLKGCELYVQLHGIQQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQKSNSQSDSHDEEVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAHLDDNERSDIFDAMFPVTHIAGETVIQQGNEGDNFYVVDQGEVDVYVNGEWVTNISEGGSFGELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQGEPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKLDRPRFERVLGPCSEILKRNIQRYNSFISLTV Predict reactive species
Full Name	protein kinase, cAMP-dependent, regulatory, type I, beta
Calculated Molecular Weight	381 aa, 43 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC026734
Gene Symbol	PRKAR1B
Gene ID (NCBI)	5575
RRID	AB_2878478
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P31321
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.