PRKCDBP Antibody
Rabbit Polyclonal
Catalog number: 16250-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | mouse |
| Positive WB detected in | mouse lung tissue, MCF7 cells |
| Positive IHC detected in | human lung cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:200-1:1000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16250-1-AP(PRKCDBP Antibody) at dilution of 1:400
Immunohistochemistry of paraffin-embedded human lung cancer using 16250-1-AP(PRKCDBP Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lung cancer using 16250-1-AP(PRKCDBP Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | PRKCDBP fusion protein ag9209 | Full name | protein kinase C, delta binding protein |
|
Calculated molecular weight |
261aa,28kd |
Observed molecular
weight |
35-40kd |
|
GenBank accession number |
BC011585 | Gene ID (NCBI) | 112464 |
| Gene symbol | PRKCDBP | Synonyms | HSRBC; MGC20400; SRBC |
Background
Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta |
