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PRMT8 Polyclonal antibody

PRMT8 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12557-1-AP

Synonyms

HRMT1L3, HRMT1L4, PRMT8

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using PRMT8 Polyclonal antibody (12557-1-AP)

    WB analysis of Y79 using 12557-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12557-1-AP (PRMT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PRMT8 Polyclonal antibody (12557-1-AP)

    IHC staining of rat brain using 12557-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRMT8 Polyclonal antibody (12557-1-AP)

    IHC staining of mouse brain using 12557-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRMT8 Polyclonal antibody (12557-1-AP)

    IHC staining of mouse brain using 12557-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRMT8 Polyclonal antibody (12557-1-AP)

    IHC staining of human breast cancer using 12557-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inY79 cells
Positive IHC detected inrat brain tissue, human breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12557-1-AP targets PRMT8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT8 fusion protein Ag3166
Full Name protein arginine methyltransferase 8
Calculated Molecular Weight 394 aa, 45 kDa
Observed Molecular Weight 43-50 kDa
GenBank Accession NumberBC022458
Gene Symbol PRMT8
Gene ID (NCBI) 56341
RRIDAB_2171964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRMT8 is a member of arginine methyl transferase (PRMT) family which acts as nuclear receptor coactivators. PRMT8 catalyzes the asymmetric arginine methylation and is involved in cellular differentiation. PRMT8 has several isoforms; the full-length isoform has a brain specific expression pattern and is localized to the plasma membrane; the truncated isoforms display nuclear localization and are expressed more widely. This antibody recognizes all of isoforms of PRMT8.

Protocols

Product Specific Protocols
WB protocol for PRMT8 antibody 12557-1-APDownload protocol
IHC protocol for PRMT8 antibody 12557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Toxicol Appl Pharmacol

Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5

Authors - Yizhen Tian
View Article
human,mouseWB

Basic Clin Pharmacol Toxicol

Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5.

Authors - Qingqing Zhang
View Article