Filter: AllWBIPIHCIF/ICC

WB analysis of human kidney using 17948-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17948-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17948-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 17948-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17948-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 17948-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 17948-1-AP
IP result of anti-PRR5 (IP:17948-1-AP, 3ug; Detection:17948-1-AP 1:300) with mouse brain tissue lysate 4000ug.
IHC staining of mouse testis using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17948-1-AP (PRR5 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17948-1-AP (PRR5 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of mouse kidney using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 17948-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 17948-1-AP
Immunofluorescent analysis of HepG2 cells, using PRR5 antibody 17948-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PRR5 Polyclonal antibody

Name: PRR5 Polyclonal antibody
Brand: Proteintech
SKU: 17948-1-AP
Rating: 5.00 (1 reviews)

PRR5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17948-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Proline-rich protein 5, Proline rich protein 5, proline rich 5 (renal), PP610, FLJ20185k

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	human kidney tissue, COLO 320 cells, HEK-293 cells, HepG2 cells, human brain tissue, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse testis tissue, mouse brain tissue, mouse kidney tissue, human kidney tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below

Product Information

17948-1-AP targets PRR5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12424 Product name: Recombinant human PRR5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-287 aa of BC016921 Sequence: MAPMPTGQKLLDSLAETWDFFFSDVLPMLQAIFYPVQGKEPSVRQLALLHFRNAITLSVKLEDALARAHARVPPAIVQMLLVLQGVHESRGVTEDYLRLETLVQKVVSPYLGTYGLHSSEGPFTHSCILEKRLLRRSRSGDVLAKNPVVRSKSYNTPLLNPVQEHEAEGAAAGGTSIRRHSVSEMTSCPEPQGFSDPPGQGPTGTFRSSPAPHSGPCPSRLYPTTQPPEQGLDPTRSSLPRSSPENLVDQILESVDSDSEGIFIDFGRGRGSGMSDLEGSGGRQSVV Predict reactive species
Full Name	proline rich 5 (renal)
Calculated Molecular Weight	31 kDa, 43 kDa
Observed Molecular Weight	33 kDa, 50 kDa
GenBank Accession Number	BC016921
Gene Symbol	PRR5
Gene ID (NCBI)	55615
RRID	AB_2268865
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P85299
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRR5, also named as PROTOR1 and PP610, is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. It acts as a tumor suppressor in breast cancer. PRR5 has some isoforms with MW 31-33 kDa and 41-50 kDa.

Protocols

Product Specific Protocols
IF protocol for PRR5 antibody 17948-1-AP	Download protocol
IHC protocol for PRR5 antibody 17948-1-AP	Download protocol
IP protocol for PRR5 antibody 17948-1-AP	Download protocol
WB protocol for PRR5 antibody 17948-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Nat Cell Biol m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. Authors - Jun Liu View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Tominica (Verified Customer) (08-19-2019)

Rabbit polyclonal anti-FUS/TLS Ab-TLS-M generated against amino acids 260–274 of mouse FUS/TLS, a stretch where the sequence is identical to the human FUS/TLS sequence. works very well.