Tested Applications
| Positive WB detected in | human kidney tissue, MCF-7 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11809-1-AP targets PSG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2468 Product name: Recombinant human PSG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 35-349 aa of BC022338 Sequence: QVTIEAEPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQMRDLYHYITSYVVDGEIIIYGPAYSGRETAYSNASLLIQNVTREDAGSYTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYRSGEV Predict reactive species |
| Full Name | pregnancy specific beta-1-glycoprotein 1 |
| Calculated Molecular Weight | 426 aa, 48 kDa |
| Observed Molecular Weight | 40-70 kDa |
| GenBank Accession Number | BC022338 |
| Gene Symbol | PSG1 |
| Gene ID (NCBI) | 5669 |
| RRID | AB_2171045 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11464 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pregnancy-specific β1-glycoproteins (PSGs) are predominant proteins synthesized during gestation in humans, primates and rodents (PMID: 5001318; 11133662). In humans, PSGs are mainly secreted by syncytiotrophoblasts, while in rodents they derive from trophoblast giant cells and spongiotrophoblasts (PMID: 9290143). Moreover, PSGs can serve as indicators for blastocyst quality and embryonic viability (PMID: 8671387; 2409861).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSG1 antibody 11809-1-AP | Download protocol |
| IHC protocol for PSG1 antibody 11809-1-AP | Download protocol |
| WB protocol for PSG1 antibody 11809-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Fertil Steril Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation. | ||
J Obstet Gynaecol Res PSG1 in Regulating Proliferation and Migration of Human Umbilical Vein Endothelial Cells Through the TGF-β/Orai3 Signaling Pathway.
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