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PSMB5 Polyclonal antibody

PSMB5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19178-1-AP

Publications (22)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:3.4.25.1, LMPX, MB1, Proteasome subunit beta type-5, X

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 19178-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19178-1-AP (PSMB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 19178-1-AP
WB result of PSMB5 antibody (19178-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 19178-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 19178-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human thyroid cancer using 19178-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human thyroid cancer using 19178-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 19178-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 19178-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 19178-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat testis using 19178-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat testis using 19178-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 19178-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19178-1-AP (PSMB5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (22)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, Jurkat cells, L02 cells
Positive IHC detected in	human thyroid cancer tissue, human breast cancer tissue, mouse brain tissue, mouse testis tissue, rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19178-1-AP targets PSMB5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5828 Product name: Recombinant human PSMB5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-263 aa of BC057840 Sequence: MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPEEPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP Predict reactive species
Full Name	proteasome (prosome, macropain) subunit, beta type, 5
Calculated Molecular Weight	263 aa, 28 kDa
Observed Molecular Weight	22 kDa
GenBank Accession Number	BC057840
Gene Symbol	PSMB5
Gene ID (NCBI)	5693
RRID	AB_10926385
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P28074
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for PSMB5 antibody 19178-1-AP	Download protocol
IHC protocol for PSMB5 antibody 19178-1-AP	Download protocol
WB protocol for PSMB5 antibody 19178-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

PSMB5 antibody (Cat# 19178-1-AP) has 22 citations across journals including Cell, Nature Communications, Oncogene, Cancer Letters, Free Radical Biology and Medicine, Analytical Chemistry, Journal of Biological Chemistry, Acta Pharmacologica Sinica, and others. Research fields span proteasome biology, multiple myeloma, breast cancer, lung cancer, bladder cancer, hepatocellular carcinoma, autophagy, cardiac hypertrophy, epilepsy, Alzheimer's disease, and eye disorders. Applications are predominantly Western blot, with some IF, IHC, and IP.

Species	Application	Title
human	WB	Cell Cancer vulnerabilities unveiled by genomic loss. Authors - Nijhawan Deepak D View Article
human	WB	Nat Commun The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma Authors - Alexander Vdovin View Article
		Cell Death Dis EphB4 inhibition defines a druggable synthetic-lethal vulnerability in MYC-driven triple-negative breast cancer. Authors - Zhe Sun View Article
human	WB,IF	Cancer Lett Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma Authors - Hongmei Luo View Article
human	WB	Cancer Lett Ixazomib triggers autophagic degradation of MUC5AC/integrin-β4 and inhibits non-small cell lung cancer progression. Authors - Muthamil Iniyan Appadurai View Article
human	WB	Phytomedicine PTEN status determines therapeutic vulnerability to celastrol in cholangiocarcinoma Authors - Yu-Fei Pan View Article