Filter: AllWBIPIHCIF/ICC

WB analysis using 24142-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24142-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24142-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 24142-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 24142-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 24142-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 24142-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 24142-1-AP
IP result of anti-PSMC3 (IP:24142-1-AP, 4ug; Detection:24142-1-AP 1:800) with HeLa cells lysate 3200ug.
IHC staining of human ovary tumor using 24142-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 24142-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human tonsillitis using 24142-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human tonsillitis using 24142-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 24142-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PSMC3 antibody (24142-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

PSMC3 Polyclonal antibody

Name: PSMC3 Polyclonal antibody
Brand: Proteintech
SKU: 24142-1-AP

PSMC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24142-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TBP-1, TBP1, Tat-binding protein 1, 26S proteasome regulatory subunit 6A, 26S proteasome AAA-ATPase subunit RPT5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, T-47D cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

24142-1-AP targets PSMC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21408 Product name: Recombinant human PSMC3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-200 aa of BC008713 Sequence: MNLLPNIESPVTRQEKMATVWDEAEQDGIGEEVLKMSTEEIIQRTRLLDSEIKIMKSEVLRVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVDPNDQEEDGANIDLDSQRKGKCAVIKTSTRQTYFLPVIGLVDAEKLKPGDLVGVNKDSYLILETLPTEYDSRVKAMEVDERPTEQYSDIGGLDKQIQELVE Predict reactive species
Full Name	proteasome (prosome, macropain) 26S subunit, ATPase, 3
Calculated Molecular Weight	49 kDa
Observed Molecular Weight	49 kDa
GenBank Accession Number	BC008713
Gene Symbol	PSMC3
Gene ID (NCBI)	5702
RRID	AB_2879440
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	P17980
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMC3(26S protease regulatory subunit 6A) is also named as TBP1, Proteasome 26S subunit ATPase 3 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. It is a multifunctional protein, component of the regulatory subunit of the proteasome, involved in different cellular processes.

Protocols

Product Specific Protocols
IF protocol for PSMC3 antibody 24142-1-AP	Download protocol
IHC protocol for PSMC3 antibody 24142-1-AP	Download protocol
IP protocol for PSMC3 antibody 24142-1-AP	Download protocol
WB protocol for PSMC3 antibody 24142-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	ChemMedChem Pyrazolones Activate the Proteasome by Gating Mechanisms and Protect Neuronal Cells from β-Amyloid Toxicity. Authors - Anna Maria Santoro View Article
human	WB	Mol Biol Cell Distinct proteostasis states drive pharmacologic chaperone susceptibility for cystic fibrosis transmembrane conductance regulator misfolding mutants Authors - Eli Fritz McDonald View Article
human	WB	Hum Mol Genet PSMC5 insufficiency and P320R mutation impair proteasome function Authors - Zhong-Qiu Yu View Article
human,mouse	WB	Elife A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression Authors - Chaojun Yan View Article