Tested Applications
| Positive WB detected in | HEK-293 cells, human kidney tissue, human heart tissue, Jurkat cells, Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
12941-1-AP targets PSMF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4011 Product name: Recombinant human PSMF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 122-271 aa of BC029836 Sequence: RTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREVDPLRIPPHHPHTSRQPPWCDPLGPFVVGGEDLDPFGPRRGGMIVDPLRSGFPRALIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPNPDHLPPPGYDDMYL Predict reactive species |
| Full Name | proteasome (prosome, macropain) inhibitor subunit 1 (PI31) |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC029836 |
| Gene Symbol | PSMF1 |
| Gene ID (NCBI) | 9491 |
| RRID | AB_2268855 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSMF1 is also named as PI31(proteasome (prosome, macropain) inhibitor subunit 1) and belongs to proteasome inhibitor PI31 family. PI31, which localizes at the nuclear envelope/endoplasmic reticulum membrane, acts as a selective modulator of the proteasome-mediated steps in MHC class I antigen processing(PMID:18495667). PI31 does not inhibit cell-surface transport of glycoproteins in general and it may serve to control immunoproteasome formation and may thereby maintain an intracellular balance between constitutive and immunoproteasomes(PMID:12374861).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMF1 antibody 12941-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Biol Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage. | ||
Mol Cell Convergence of orphan quality control pathways at a ubiquitin chain-elongating ligase |
