Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, HepG2 cells, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10472-1-AP targets PTPN23 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0765 Product name: Recombinant human PTPN23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 420-619 aa of BC004881 Sequence: QEKLHLRFCYEAVVRHVEQVLQRHGVPPPCKPLASASISQKNHLPQDSQDLVLGGDVPISSIQATIAKLSIRPPGGLESPVASLPGPAEPPGLPPASLPESTPIPSSSPPPLSSPLPEAPQPKEEPPVPEAPSSGPPSSSLELLASLTPEAFSLDSSLRGKQRMSKHNFLQAHNGQGLRATRPSDDPLSLLDPLWTLNKT Predict reactive species |
| Full Name | protein tyrosine phosphatase, non-receptor type 23 |
| Calculated Molecular Weight | 179 kDa |
| Observed Molecular Weight | 179-200 kDa |
| GenBank Accession Number | BC004881 |
| Gene Symbol | PTPN23 |
| Gene ID (NCBI) | 25930 |
| RRID | AB_2173382 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3S7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTPN23 antibody 10472-1-AP | Download protocol |
| IHC protocol for PTPN23 antibody 10472-1-AP | Download protocol |
| IP protocol for PTPN23 antibody 10472-1-AP | Download protocol |
| WB protocol for PTPN23 antibody 10472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PTPN23 Polyclonal antibody (Cat# 10472-1-AP) has 19 citations across journals including Nature Communications, Cancer Letters, Developmental Cell, EMBO Journal, Current Biology, Cell Reports, Genes & Development, Journal of Cell Biology, PLoS Pathogens, Structure, iScience, Journal of Biological Chemistry, and Journal of Cell Science. Research fields span ESCRT-mediated endosomal sorting, multivesicular body formation, lysosome repair, microautophagy, exosome biogenesis, tumor biology (esophageal, breast, testicular cancers), EGFR/CTLA4 signaling, HIV-1 Vpu trafficking, and TGFβ pathway regulation.
| Species | Application | Title |
|---|---|---|
Nat Commun Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation. | ||
Cancer Lett PTPN23[Thr] variant reduces susceptibility and tumorigenesis in esophageal squamous cell carcinoma through dephosphorylation of EGFR | ||
Dev Cell Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies
| ||
Curr Biol Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.
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