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WB analysis using 12575-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12575-1-AP
IP result of anti-PTTG1IP (IP:12575-1-AP, 4ug; Detection:12575-1-AP 1:300) with HEK-293 cells lysate 2600 ug.
IHC staining of human testis using 12575-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12575-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 12575-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 12575-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 12575-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 12575-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 12575-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTTG1IP antibody (12575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of PC-3 using 12575-1-AP
Immunofluorescent analysis of PC-3 cells using 12575-1-AP (PTTG1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PTTG1IP Polyclonal antibody

Name: PTTG1IP Polyclonal antibody
Brand: Proteintech
SKU: 12575-1-AP

PTTG1IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12575-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PTTG-binding factor, Pituitary tumor-transforming gene protein-binding factor, Pituitary tumor-transforming gene 1 protein-interacting protein, C21orf3, C21orf1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A375 cells, HEK-293 cells, HeLa cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human testis tissue, human colon tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below

Product Information

12575-1-AP targets PTTG1IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3250 Product name: Recombinant human PTTG1IP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC012858 Sequence: MAPGVARGPTPYWRLRLGGAALLLLLIPVAAAQEPPGAACSQNTNKTCEECLKNVSCLWCNTNKACLDYPVTSVLPPASLCKLSSARWGVCWVNFEALIITMSVVGGTLLLGIAICCCCCCRRKRSRKPDRSEEKAMREREERRIRQEERRAEMKTRHDEIRKKYGLFKEENPYARFENN Predict reactive species
Full Name	pituitary tumor-transforming 1 interacting protein
Calculated Molecular Weight	180 aa, 20 kDa
Observed Molecular Weight	26 kDa
GenBank Accession Number	BC012858
Gene Symbol	PTTG1IP
Gene ID (NCBI)	754
RRID	AB_10644145
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P53801
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for PTTG1IP antibody 12575-1-AP	Download protocol
IHC protocol for PTTG1IP antibody 12575-1-AP	Download protocol
IP protocol for PTTG1IP antibody 12575-1-AP	Download protocol
WB protocol for PTTG1IP antibody 12575-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Horm Metab Res MicroRNA-524-5p Functions as a Tumor Suppressor in a Human Pituitary Tumor-Derived Cell Line. Authors - Wang Zhen View Article
human	IHC	Oncol Lett Expression of proteins associated with epithelial-mesenchymal transition in esophageal squamous cell carcinoma. Authors - Tian Zhang View Article
human	WB	Pharmacol Res PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma Authors - Wenfang Bao View Article