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PVRIG Polyclonal antibody

PVRIG Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21448-1-AP

Synonyms

C7orf15, CD112R, PVRIG, Transmembrane protein PVRIG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PVRIG Polyclonal antibody (21448-1-AP)

    WB analysis using 21448-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21448-1-AP (PVRIG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PVRIG Polyclonal antibody (21448-1-AP)

    IHC staining of human tonsillitis using 21448-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PVRIG Polyclonal antibody (21448-1-AP)

    IHC staining of human tonsillitis using 21448-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMOLT-4 cells, NK-92 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21448-1-AP targets PVRIG in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PVRIG fusion protein Ag13815
Full Name poliovirus receptor related immunoglobulin domain containing
Calculated Molecular Weight 326 aa, 34 kDa
Observed Molecular Weight ~34 kDa
GenBank Accession NumberBC001129
Gene Symbol PVRIG
Gene ID (NCBI) 79037
RRIDAB_2878859
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PVRIG, also named as CD112R and C7orf15, is an immune suppressive molecule expressed on T and NK cells. It's a cell surface receptor for NECTIN2. PVRIG is a target for the treatment of cancer.

Protocols

Product Specific Protocols
WB protocol for PVRIG antibody 21448-1-APDownload protocol
IHC protocol for PVRIG antibody 21448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols