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PWP1 Polyclonal antibody

PWP1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 14767-1-AP

Synonyms

IEF SSP 9502, PWP1, PWP1 homolog (S. cerevisiae)

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PWP1 Polyclonal antibody (14767-1-AP)

    WB analysis of Jurkat using 14767-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14767-1-AP (PWP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PWP1 Polyclonal antibody (14767-1-AP)

    WB analysis of human liver using 14767-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14767-1-AP (PWP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PWP1 Polyclonal antibody (14767-1-AP)

    WB analysis of Jurkat using 14767-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14767-1-AP (PWP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, human liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14767-1-AP targets PWP1 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PWP1 fusion protein Ag6533
Full Name PWP1 homolog (S. cerevisiae)
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC001652
Gene Symbol PWP1
Gene ID (NCBI) 11137
RRIDAB_2174294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PWP1 antibody 14767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

H (Verified Customer) (04-05-2020)

There are quite lots of background bands. However, I could detect which is the target band through nuclear-cytosolic fractionation under starvation condition, during which, PWP1 translocate from nucleus to cytosol.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
PWP1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14767-1-AP)