PYCR1 Antibody
Rabbit Polyclonal
Catalog number: 13108-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IF, WB |
| Species specificity | human, mouse, rat, zebrafish; other species not tested. |
| Cited species | human, zebrafish |
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human prostate cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
human brain tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP(PYCR1 Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human prostate cancer using 13108-1-AP(PYCR1 Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using PYCR1 antibody 13108-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | PYCR1 fusion protein ag3764 | Full name | pyrroline-5-carboxylate reductase 1 |
|
Calculated molecular weight |
319aa,33.8kd |
Observed molecular
weight |
33.8kd |
|
GenBank accession number |
BC022244 | Gene ID (NCBI) | 5831 |
| Gene symbol | PYCR1 | Synonyms | P5C; P5CR; PIG45; PP222; PYCR |
Background
PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. |
