PYCR1 Antibody
Rabbit Polyclonal
Catalog number: 20962-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | Raji cells |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP(PYCR1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using 20962-1-AP(PYCR1 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer using 20962-1-AP(PYCR1 Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Peptide | Full name | pyrroline-5-carboxylate reductase 1 |
|
Calculated molecular weight |
33kd |
Observed molecular
weight |
34kd |
|
GenBank accession number |
NM_006907 | Gene ID (NCBI) | 5831 |
| Gene symbol | PYCR1 | Synonyms | P5C; P5CR; PIG45; PP222; PYCR |
Background
PYCR1, also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is involved in the cellular response to oxidative stress. PYCR1 catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). The antibody is specific to PYCR1. It has no cross reaction to PYCR2. |
