Filter: AllWBIHCIF/ICC

WB analysis using 17146-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 17146-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17146-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17146-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 17146-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17146-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 17146-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 17146-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR2 antibody (17146-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PYCR2 Polyclonal antibody

Name: PYCR2 Polyclonal antibody
Brand: Proteintech
SKU: 17146-1-AP
Rating: 4.75 (4 reviews)

PYCR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17146-1-AP

Publications(26)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:1.5.1.2, HLD10, P5CR 2, P5CR2, PYCR 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (26)
Protocols
Reviews (4)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells, L02 cells
Positive IHC detected in	human breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 20 publications below
IHC	See 3 publications below
IF	See 3 publications below

Product Information

17146-1-AP targets PYCR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, african green monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10918 Product name: Recombinant human PYCR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC020553 Sequence: MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD Predict reactive species
Full Name	pyrroline-5-carboxylate reductase family, member 2
Calculated Molecular Weight	320 aa, 34 kDa
Observed Molecular Weight	34 kDa
GenBank Accession Number	BC020553
Gene Symbol	PYCR2
Gene ID (NCBI)	29920
RRID	AB_2253344
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96C36
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

Protocols

Product Specific Protocols
IF protocol for PYCR2 antibody 17146-1-AP	Download protocol
IHC protocol for PYCR2 antibody 17146-1-AP	Download protocol
WB protocol for PYCR2 antibody 17146-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma. Authors - Qiang Gao View Article
human	WB	Sci Adv Loss of attachment promotes proline accumulation and excretion in cancer cells Authors - Steven E Pilley View Article
mouse	WB	EMBO J Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress. Authors - Simon Schwörer View Article
human	WB	EMBO J Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis. Authors - Zhen Chen View Article
human	WB	Cell Rep Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis. Authors - Kate E R Hollinshead KD Validated View Article
African green monkey	WB	Virol Sin African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2. Authors - Sheng Chen KD Validated View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH balawant (Verified Customer) (12-25-2023)	I have used this antibody . This antibody is working great. Applications: Western Blot, Immunohistochemistry Primary Antibody Dilution: 1:1000 for western blot and 1:700 for IHC Cell Tissue Type: colon tissue of mice and human
FH Balawant (Verified Customer) (08-06-2022)	This antibody is working great. I have used this antibody for tissue lysate and cell lysate. it has no background. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: colon tissue and colon cancer cells
FH Susmita (Verified Customer) (03-11-2022)	Excellent antibody Applications: Western Blot Primary Antibody Dilution: WB: 1: 2000 Cell Tissue Type: CRC cell line
FH Iram (Verified Customer) (07-03-2020)	Giving a very sharp and clean result Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human