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  • Featured Product
  • KD/KO Validated

ProSAPiP1 Polyclonal antibody

ProSAPiP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24936-1-AP

Synonyms

KIAA0552, ProSAP interacting protein 1, ProSAPiP1, ProSAPiP1 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ProSAPiP1 Polyclonal antibody (24936-1-AP)

    WB analysis using 24936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ProSAPiP1 Polyclonal antibody (24936-1-AP)

    IHC staining of rat brain using 24936-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ProSAPiP1 Polyclonal antibody (24936-1-AP)

    IHC staining of mouse brain using 24936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24936-1-AP is ProSAPiP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ProSAPiP1 fusion protein Ag20765
Full Name ProSAPiP1 protein
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC038860
Gene Symbol ProSAPiP1
Gene ID (NCBI) 9762
RRIDAB_2714022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ProSAPiP1 antibody 24936-1-APDownload protocol
IHC protocol for ProSAPiP1 antibody 24936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Neuron

Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward.

Authors - Sophie Laguesse
  • KD Validated
View Article