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KD/KO Validated

GlnRS Polyclonal antibody

GlnRS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12645-1-AP

Publications(10)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

QARS, EC:6.1.1.18, Glutamine--tRNA ligase, Glutaminyl-tRNA synthetase, QARS1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

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Quantity

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Proteintech Guarantee

Filter: AllWBIPIHCIF/ICC

WB analysis using 12645-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 12645-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of NIH/3T3 using 12645-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-3 using 12645-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 12645-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 12645-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-3 using 12645-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of PC-3 using 12645-1-AP
IP result of anti-GlnRS (IP:12645-1-AP, 4ug; Detection:12645-1-AP 1:500) with PC-3 cells lysate 3200ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate cancer using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate cancer using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat ovary using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat stomach using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of PC-3 using 12645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GlnRS antibody (12645-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 12645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GlnRS antibody (12645-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (10)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, NIH/3T3 cells, PC-3 cells
Positive IP detected in	PC-3 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:1500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12645-1-AP targets GlnRS in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3375 Product name: Recombinant human QARS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 362-717 aa of BC016634 Sequence: VKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV Predict reactive species
Full Name	glutaminyl-tRNA synthetase
Calculated Molecular Weight	775 aa, 88 kDa
Observed Molecular Weight	88 kDa
GenBank Accession Number	BC016634
Gene Symbol	GlnRS
Gene ID (NCBI)	5859
RRID	AB_2098676
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P47897
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).

Protocols

Product Specific Protocols
IF protocol for GlnRS antibody 12645-1-AP	Download protocol
IHC protocol for GlnRS antibody 12645-1-AP	Download protocol
IP protocol for GlnRS antibody 12645-1-AP	Download protocol
WB protocol for GlnRS antibody 12645-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Protein Cell Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation Authors - Kai-Hui Zhang View Article
human	WB	Nat Cell Biol Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics. Authors - Maria C Passarelli View Article
human	WB	Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation Authors - Raja H Ali View Article
human	WB	Nucleic Acids Res Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex. Authors - Haissi Cui View Article
human	WB,RIP	EMBO Rep The tRNA-like small noncoding RNA mascRNA promotes global protein translation. Authors - Xinping Lu KD Validated View Article
	WB	J Physiol Biochem Molecular biomarkers for the prognosis of breast cancer: role of amino acid metabolism genes Authors - Yudong Zhou View Article