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  • Featured Product
  • KD/KO Validated

GlnRS Polyclonal antibody

GlnRS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12645-1-AP

Synonyms

GlnRS, Glutamine tRNA ligase, glutaminyl tRNA synthetase, PRO2195, QARS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GlnRS Polyclonal antibody (12645-1-AP)

    WB analysis using 12645-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GlnRS Polyclonal antibody (12645-1-AP)

    WB analysis of HepG2 using 12645-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GlnRS Polyclonal antibody (12645-1-AP)

    WB analysis of PC-3 using 12645-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using GlnRS Polyclonal antibody (12645-1-AP)

    WB analysis of NIH/3T3 using 12645-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GlnRS Polyclonal antibody (12645-1-AP)

    WB analysis of HeLa using 12645-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GlnRS Polyclonal antibody (12645-1-AP)

    WB analysis of PC-3 using 12645-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GlnRS Polyclonal antibody (12645-1-AP)

    WB analysis of HeLa using 12645-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using GlnRS Polyclonal antibody (12645-1-AP)

    IP experiment of PC-3 using 12645-1-AP

    IP result of anti-GlnRS (IP:12645-1-AP, 4ug; Detection:12645-1-AP 1:500) with PC-3 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GlnRS Polyclonal antibody (12645-1-AP)

    IHC staining of human prostate cancer using 12645-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GlnRS Polyclonal antibody (12645-1-AP)

    IHC staining of human prostate cancer using 12645-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using GlnRS Polyclonal antibody (12645-1-AP)

    IF Staining of PC-3 using 12645-1-AP

    Immunofluorescent analysis of PC-3 cells using 12645-1-AP (GlnRS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:1500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12645-1-AP targets GlnRS in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GlnRS fusion protein Ag3375
Full Name glutaminyl-tRNA synthetase
Calculated Molecular Weight 775 aa, 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC016634
Gene Symbol QARS
Gene ID (NCBI) 5859
RRIDAB_2098676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).

Protocols

Product Specific Protocols
WB protocol for GlnRS antibody 12645-1-APDownload protocol
IHC protocol for GlnRS antibody 12645-1-APDownload protocol
IF protocol for GlnRS antibody 12645-1-APDownload protocol
IP protocol for GlnRS antibody 12645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.

Authors - Maria C Passarelli
View Article
humanWB

Nucleic Acids Res

Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.

Authors - Haissi Cui
View Article
humanWB,RIP

EMBO Rep

The tRNA-like small noncoding RNA mascRNA promotes global protein translation.

Authors - Xinping Lu
  • KD Validated
View Article