RAB11FIP1 Polyclonal antibody
RAB11FIP1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey
Applications
WB, RIP, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16778-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, COS-7 cells, SKOV-3 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| RIP | See 2 publications below |
Product Information
16778-1-AP targets RAB11FIP1 in WB, RIP, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB11FIP1 fusion protein Ag10179 |
| Full Name | RAB11 family interacting protein 1 (class I) |
| Calculated Molecular Weight | 649aa,71 kDa; 1283aa,137 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC077720 |
| Gene Symbol | RAB11FIP1 |
| Gene ID (NCBI) | 80223 |
| RRID | AB_2878315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB11FIP1 antibody 16778-1-AP | Download protocol |
| IHC protocol for RAB11FIP1 antibody 16778-1-AP | Download protocol |
| IF protocol for RAB11FIP1 antibody 16778-1-AP | Download protocol |
| IP protocol for RAB11FIP1 antibody 16778-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein | ||
Brief Bioinform mi-IsoNet: systems-scale microRNA landscape reveals rampant isoform-mediated gain of target interaction diversity and signaling specificity. | ||
Cancer Res CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942. | ||
Am J Cancer Res In silico development and in vitro validation of a novel five-gene signature for prognostic prediction in colon cancer |
