Tested Applications
| Positive WB detected in | HT-29 cells, Caco-2 cells, T-47D cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
13189-1-AP targets RAB25 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3864 Product name: Recombinant human RAB25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-213 aa of BC033322 Sequence: MGNGTEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAVKAQIWDTAGLERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQAREVPTEEARMFAENNGLLFLETSALDSTNVELAFETVLKEIFAKVSKQRQNSIRTNAITLGSAQAGQEPGPGEKRACCISL Predict reactive species |
| Full Name | RAB25, member RAS oncogene family |
| Calculated Molecular Weight | 213 aa, 23 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC033322 |
| Gene Symbol | RAB25 |
| Gene ID (NCBI) | 57111 |
| RRID | AB_2877921 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P57735 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAB25 antibody 13189-1-AP | Download protocol |
| WB protocol for RAB25 antibody 13189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FEBS J The protease ADAMTS5 controls ovarian cancer cell invasion, downstream of Rab25
| ||
Nat Commun Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane
| ||
Transl Cancer Res Rab25 acts as an oncogene and participates in the regulation of aerobic glycolysis via PKM2 in gastric adenocarcinoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Shan (Verified Customer) (10-20-2025) | Product came very fast (delivered the next day).
|
FH Elena (Verified Customer) (05-13-2022) | The antibody detected a single band at the expected molecular weight, under both reducing and non-reducing conditions
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