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  • KD/KO Validated

RAB31-specific Polyclonal antibody

RAB31-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16182-1-AP

Synonyms

Rab22B, RAB31, RAB31-specific, Ras related protein Rab 22B, Ras related protein Rab 31



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, K-562 cells
Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16182-1-AP targets RAB31-specific in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB31, member RAS oncogene family
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_006868
Gene Symbol RAB31
Gene ID (NCBI) 11031
RRIDAB_1851568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB 31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes. This antibody can bind RAB31 specifically, and not react RAB22a.

Protocols

Product Specific Protocols
WB protocol for RAB31-specific antibody 16182-1-APDownload protocol
IHC protocol for RAB31-specific antibody 16182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
  • KD Validated
humanWB

Int J Biol Sci

Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation.

Authors - Yujie Huang
  • KD Validated
humanWB,IHC,CoIP

Front Oncol

RAB31 Targeted by MiR-30c-2-3p Regulates the GLI1 Signaling Pathway, Affecting Gastric Cancer Cell Proliferation and Apoptosis.

Authors - Chao-Tao Tang
  • KD Validated
humanWB

PLoS One

Human liver cell trafficking mutants: characterization and whole exome sequencing.

Authors - Fei Yuan
human,mouseWB,IHC,IF

Int J Biochem Cell Biol

Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis.

Authors - Chan Qiu
  • KD Validated
humanWB,IHC

Life Sci

Upregulation of Rab31 is associated with poor prognosis and promotes colorectal carcinoma proliferation via the mTOR/p70S6K/Cyclin D1 signalling pathway.

Authors - Li Yang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Edward (Verified Customer) (11-15-2022)

Showed up reasonably well using 12 ug protein lysate with fluorescent secondary

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: K562, KCL22 and THP-1